Andrea Doni is the Head of the Imaging Unit at IRCCS Humanitas Research Hospital.

Doni obtained a PhD in Immunology and Immunopathology from the Open University of London. From 1995 to 2005, he has been working in the Department of Immunology directed by Professor Alberto Mantovani at Pharmacological Institute Mario Negri of Milan, before moving in 2005 at IRCCS Humanitas Research Hospital.

He is specialized in studies of preclinical research with specific expertise in experimental in vivo models of humoral resistance against pathogens, in prognostic and predictive biomarker discovery of inflammatory diseases, involvement of components of the innate immune system in tissue repair. He contributed to define the regulation and the in vivo roles of the innate immune molecules, PTX3 and SAP. He is active in studies concerning the influence of the microenvironment in the progression of the tumor, with particular focus on the factors involved in promoting the adhesion and the invasiveness of cancer cells. He developed several original reagents, including monoclonal antibodies and ELISA, for PTX3 and other molecules of interest in the lab, including MCP-1, IL-1ra, icIL-1ra, MSF.

Since 2002, he deals with advanced optical microscopy. He maintains and develops optical technologies for basic and advanced research, in linear (wide field fluorescence; Live Cell Imaging; confocal microscopy; FRAP, FLIP, TIRF, Photoactivation; Photoconversion; Uncaging) and non-linear (super-resolution STED, FLIM-based FRET, 2P excitation) microscopy. He developed different protocols and in vitro and in vivo experimental models for confocal and intravital optical microscopy analysis. His further current interest is in multiparametric spatial analysis of tissue using Imaging Mass Cytometry systems.

He contributed as first author or co-author in several scientific publications in international peer-reviewed journals. (Orcid 0000-0001-9203-5814; Scopus ID 7004747845; H-index= 56).