RESEARCH GROUP

Stem Cell

 

The Stem Cell Unit is a “joint laboratory” which main aim is to develop new approaches for dissecting mechanisms of human diseases and to accelerate development of new therapies.

The Unit brings together researchers withexpertise in different fields of stem cells and developmental biology, from reprogramming to differentiation into specific lineages, gene-editing strategies and chromosome transplantation, and thus providing a solid ground for a comprehensive investigation of mechanisms of human diseases in vitro and for advanced stem cell biology and translational medicine projects.

Induced pluripotent stem cells (iPSCs) applied to cardiac diseases (Dr. Di Pasquale).

This line of research aims to unveil new mechanisms at the basis of cardiac diseases for the development of more effective therapeutic strategies. To this goal, we integrate electrophysiological, molecular and imaging techniques and genome-editing strategies.

CRISPR/Cas9-based genome editing strategies for diseases’ investigation and therapy (Drs Di Pasquale and Paulis).

This area of research is dedicated to the development of “customized” genome-editing tools for translational application of iPSC-based models to specifically target genes or sequences of interest in mouse and human pluripotent stem cells.

Pluripotent Stem cell-based genomic therapy (Dr. Paulis). 

The research is addressed to genomic disorders due to chromosome abnormalities based on the replacement of defective chromosomes (Chromosome Transplantation) in human patient-specific iPSCs, for which conventional gene therapy is still unsatisfactory.

 

Methodologies

Generation, cultivation and characterization of mouse and human patient-specific iPSCs, their genome manipulation as well as their in vitro differentiation into relevant cell types (as cardiomyocytes and haematopoietic lineages) are the main technical approaches applied in the laboratory.

We have already generated and available lines from patients affected with different diseases (Laminopathies and other forms of familial Dilated Cardiomyopathy, various types of Inherited Arrhythmias; Lesch Nyhan, Turner, Duchenne and Down syndromes, Osteopetrotic disorder) and from control donors.

We also have pluripotent stem cells from different mouse models (ES from Lesch Nyhan model: HM1 and E14tg2a; iPSCs from osteopetrotic mouse (oc/oc-iPSCs) and from Chronic Granulomatous Disease (CGD-iPSCs).

Other activities

The laboratory is also collaborating as support on new projects and it is active in the continuous development of cutting-edge technologies and in the organization of basic training programs and topic-dedicated workshops.

    Selected Publications

  • Elisa Di Pasquale Publications

    Selected publications: Di Mauro, Crasto S, Colombo FS, Di Pasquale E, Catalucci D. 2019. Wnt signalling  mediates miR-133a muclear re-localization for the transcriptional control of Dnmt3b in cardiac cells. Sci ...

  • Marianna Paulis Publications

    Salvarani N., Crasto S., Miragoli M., Bertero A., Paulis M., Kunderfranco P., Serio S., Forni A., Lucarelli C., Dal Ferro M., Larcher V., Sinagra G., Vezzoni P., Murry C.E., Faggian ...

Group Members