About the Course

Optical Microscopy: From Epifluorescence to Multiphoton Microscopy

This course is designed for research scientist working in biological sciences. The aim of this course is to bridge the gap between the theory on Fluorescence Microscopy and daily usage.

The course will be held in English.

Day 1

9.30-12.30 Basics of Fluorescence Microscopy

• Introduction to microscopy
• Basic aspects of optical microscopy
• Principles of Fluorescence
• Confocal versus widefield fluorescence microscopy

Optical bench demonstrations will be used throughout the course to explain the main topics

14-15.30 Application

• Samples preparation
• Fluorophores
• Spectral bleed-through artefacts
• XY, XYZ, XYT, XYλ acquisitions
• Live cell imaging

15.30-16.30 Advanced Techniques in Linear Microscopy

• TIRF (Total Internal Reflection)
• FRAP (Fluorescence Recovery after Photo-Bleaching) / FLIP (Fluorescence Loss in Photo-Bleaching)
• FRET (Fluorescence Resonance Energy Transfer)

Day 2

9.00-9.45 In Vivo imaging

9.45-11.30 Non-Linear Microscopy

• Two Photon Microscopy
• FLIM (Fluorescence Lifetime Imaging)
• Intravital microscopy

11.30-13.00 Frontiers of Microscopy

• Super-resolution
• Light Sheet Microscopy
• Principles of Clarification Techniques

14.00-16.00 Practical introduction to Instruments 

Fees